| Supplementary Table 5. |
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| Microarray
results of 49 "differentially regulated" probes identified by SAM
and 2-way ANOVA a. |
|
| Spot ID |
Gene |
GenBank |
UniGene |
ANOVA b |
Class c |
Function |
biological process |
Molecular function |
Cellular component |
GeneSymbol |
GripQuery |
Description |
V_6h vs NT |
V_24h vs NT |
V_72h vs NT |
L_6h vs NT |
L_24h vs NT |
L_72h vs NT |
H_6h vs NT |
H_24h vs NT |
H_72h vs NT |
|
| 894280 |
Mphosph6 |
NM_026758 |
Mm.181836 |
MABx |
403 |
cell cycle |
M phase of mitotic cell cycle;regulation of cell cycle |
|
nucleus |
Mphosph6 |
NM_026758 |
M phase phosphoprotein 6 |
0.68 |
-0.06 |
-0.66 |
0.67 |
-0.28 |
-1.03 |
1.12 |
0.34 |
-0.78 |
|
| 527912 |
Abi3 |
BC035943 |
Mm.129228 |
MABx |
101 |
cell migration |
regulation of cell migration |
molecular function unknown |
cellular component unknown |
Phospho1 |
BC035943 |
phosphatase, orphan 1 |
-0.51 |
-0.26 |
0.29 |
-1.00 |
-0.50 |
0.00 |
-2.40 |
-1.23 |
-0.18 |
|
| 901543 |
Hist1h2ao |
NM_178185 |
Mm.342416 |
MxBx |
913 |
chromosome organization
and biogenesis |
Hist1h2ao |
NM_178185 |
histone 1, H2ao |
-0.48 |
-0.48 |
-0.57 |
-1.30 |
-0.61 |
-0.33 |
-1.32 |
0.35 |
-0.03 |
|
| 704890 |
Micl |
NM_177686 |
Mm.90241 |
MAxx |
101 |
immune response |
regulation of immune response |
protein phosphatase binding;sugar binding |
cell surface |
D230024O04 |
NM_177686 |
hypothetical protein D230024O04 |
-0.25 |
0.42 |
0.43 |
0.24 |
0.83 |
-0.22 |
1.71 |
0.97 |
0.68 |
|
| 931440 |
Prkab1 |
NM_031869 |
Mm.200912 |
MxBx |
206 |
metabolism (carbohydrate ) |
carbohydrate metabolism;fatty acid biosynthesis;lipid
biosynthesis;response to stress |
AMP-activated protein kinase activity;hydrolase activity,
hydrolyzing O-glycosyl compounds |
nucleus |
Prkab1 |
NM_031869 |
protein kinase, AMP-activated, beta 1 non-catalytic subunit |
0.54 |
0.34 |
-0.12 |
1.09 |
0.56 |
-0.37 |
1.22 |
0.59 |
-0.14 |
|
| 551010 |
Pafah1b3 |
NM_008776 |
Mm.597 |
MxBI |
103 |
metabolism (lipid) |
lipid catabolism;spermatogenesis;spermatogenesis |
1-alkyl-2-acetylglycerophosphocholine esterase
activity;catalytic activity;hydrolase activity |
cytoplasm |
Pafah1b3 |
NM_008776 |
platelet-activating factor acetylhydrolase, isoform 1b, alpha1
subunit |
-0.67 |
-0.03 |
-0.29 |
-2.48 |
0.22 |
-0.47 |
-3.14 |
0.37 |
-0.24 |
|
| 735288 |
Nme4 |
BC027277 |
Mm.41787 |
MxBx |
712 |
metabolism (nucleotide) |
CTP biosynthesis;GTP biosynthesis;UTP biosynthesis;nucleotide
metabolism |
ATP binding;kinase activity;magnesium ion
binding;nucleoside-diphosphate kinase activity;nucleotide binding;transferase
activity |
extracellular space;mitochondrion |
Nme4 |
BC027277 |
expressed in non-metastatic cells 4, protein |
0.43 |
0.26 |
-0.57 |
-1.92 |
-0.22 |
-0.41 |
-0.96 |
0.06 |
-1.12 |
|
| 494447 |
9230110J10 |
NM_183301 |
Mm.307932 |
MABx |
204 |
metabolism (nucleotide) |
|
E2f2;9230110J10 |
NM_183301 |
E2F transcription factor 2;hypothetical protein 9230110J10 |
-0.42 |
0.38 |
-0.53 |
-1.78 |
-0.58 |
-1.50 |
-1.17 |
1.06 |
-0.33 |
|
| 551096 |
Cds2 |
NM_138651 |
Mm.284503 |
MABI |
102 |
metabolism (phospholipid) |
phospholipid biosynthesis;phospholipid metabolism |
nucleotidyltransferase activity;phosphatidate
cytidylyltransferase activity;transferase activity |
integral to membrane;membrane;mitochondrial inner membrane |
Cds2 |
NM_138651 |
CDP-diacylglycerol synthase (phosphatidate
cytidylyltransferase) 2 |
-0.66 |
-0.46 |
-0.48 |
-1.90 |
-0.15 |
-0.59 |
-0.91 |
0.36 |
-0.58 |
|
| 631716 |
Lsm8 |
NM_133939 |
Mm.275158 |
MABI |
102 |
mRNA processing |
mRNA processing;nuclear mRNA splicing, via spliceosome |
RNA binding;U6 snRNA binding |
nucleus;ribonucleoprotein complex;small nucleolar
ribonucleoprotein complex |
Lsm8 |
NM_133939 |
LSM8 homolog, U6 small nuclear RNA associated (S. cerevisiae) |
-0.40 |
-0.30 |
-0.53 |
-0.98 |
-0.18 |
-0.28 |
-0.94 |
0.31 |
-0.34 |
|
| 924677 |
Leng5 |
NM_024168 |
Mm.379100 |
MxBx |
1312 |
mRNA processing |
mRNA processing;tRNA processing;tRNA splicing |
endonuclease activity;hydrolase activity;nuclease
activity;receptor activity;tRNA-intron endonuclease activity |
nucleus;tRNA-intron endonuclease complex |
Leng5 |
NM_024168 |
leukocyte receptor cluster (LRC) member 5 |
-0.50 |
-0.18 |
-0.16 |
-0.87 |
-0.27 |
-0.24 |
-0.51 |
-0.26 |
-0.28 |
|
| 812600 |
C730042F17Rik |
NM_145517 |
Mm.386860 |
MxBx |
1311 |
protein targeting |
protein targeting |
|
integral to membrane |
C730042F17Rik |
NM_145517 |
RIKEN cDNA C730042F17 gene |
-0.61 |
-0.12 |
0.33 |
-0.82 |
-0.24 |
0.13 |
-1.15 |
0.14 |
-0.13 |
|
| 772594 |
P2ry12 |
NM_027571 |
Mm.386801 |
MABI |
102 |
signal transduction |
G-protein coupled receptor protein signaling pathway;G-protein
signaling, coupled to cAMP nucleotide second messenger;platelet
activation;signal transduction |
G-protein coupled receptor activity;adenosine receptor
activity, G-protein coupled;platelet ADP receptor activity;purinergic
nucleotide receptor activity, G-protein coupled;receptor
activity;rhodopsin-like receptor activity;signal transducer activity |
integral to membrane;integral to plasma membrane |
P2ry12 |
NM_027571 |
purinergic receptor P2Y, G-protein coupled 12 |
0.03 |
-0.35 |
0.42 |
-0.18 |
0.18 |
0.09 |
0.40 |
1.62 |
-0.11 |
|
| 822012 |
Ltb4r2 |
NM_020490 |
Mm.159670 |
MxBx |
1209 |
signal transduction |
G-protein coupled receptor protein signaling pathway;signal
transduction |
G-protein coupled receptor activity;leukotriene receptor
activity;receptor activity;rhodopsin-like receptor activity;signal transducer
activity |
integral to membrane |
Ltb4r2 |
NM_020490 |
leukotriene B4 receptor 2 |
-0.39 |
0.30 |
-0.53 |
-1.03 |
-0.29 |
0.15 |
-1.74 |
-0.23 |
-0.03 |
|
| 374634 |
Tec |
NM_013689 |
Mm.319581 |
MxBx |
810 |
signal transduction |
intracellular signaling cascade;protein amino acid
phosphorylation |
ATP binding;kinase activity;protein binding;protein kinase
activity;protein serine/threonine kinase activity;protein-tyrosine kinase
activity;transferase activity |
|
Tec |
NM_013689 |
cytoplasmic tyrosine kinase, Dscr28C related (Drosophila) |
-0.64 |
-0.11 |
-0.06 |
-2.15 |
-0.02 |
-0.08 |
-0.74 |
-0.38 |
-0.24 |
|
| 924253 |
Sh2d3c |
NM_013781 |
Mm.9593 |
MABI |
101 |
signal transduction |
intracellular signaling cascade;signal transduction |
guanyl-nucleotide exchange factor activity;receptor
activity;transmembrane receptor protein tyrosine kinase adaptor protein
activity |
|
Sh2d3c |
NM_013781 |
SH2 domain containing 3C |
0.01 |
-0.05 |
0.14 |
-0.35 |
-0.13 |
0.44 |
-1.23 |
-0.27 |
-0.23 |
|
| 928204 |
Hs1bp3 |
NM_021429 |
Mm.309954 |
MABI |
102 |
signal transduction |
T cell differentiation;cell surface receptor linked signal
transduction;intracellular signaling cascade |
protein binding |
cellular component unknown |
Hs1bp3 |
NM_021429 |
HS1 binding protein 3 |
-0.33 |
-0.09 |
-0.16 |
-1.45 |
-0.42 |
-0.34 |
-0.61 |
0.01 |
-0.56 |
|
| 774463 |
Wisp1 |
NM_018865 |
Mm.10222 |
MxBx |
1210 |
signal transduction |
Wnt receptor signaling pathway;cell adhesion;regulation of
cell growth |
insulin-like growth factor binding;protein binding |
extracellular region;extracellular space |
Wisp1 |
NM_018865 |
WNT1 inducible signaling pathway protein 1 |
-0.28 |
-0.61 |
0.17 |
-2.26 |
-0.48 |
-0.01 |
-1.36 |
0.09 |
0.22 |
|
| 642099 |
Plxnb3 |
NM_019587 |
Mm.380177 |
MxBx |
1007 |
signal transduction |
|
Plxnb3 |
NM_019587 |
plexin B3 |
-0.13 |
-0.48 |
0.22 |
-1.13 |
-0.44 |
-0.22 |
-0.70 |
-0.48 |
0.09 |
|
| 900157 |
Hmgb3 |
NM_008253 |
Mm.336087 |
MxBx |
1110 |
transcription |
DNA packaging;negative regulation of B cell
differentiation;negative regulation of myeloid cell
differentiation;regulation of transcription, DNA-dependent |
DNA binding |
chromatin;chromosome;nucleus |
Hmgb3 |
NM_008253 |
high mobility group box 3 |
-0.44 |
-0.61 |
-0.37 |
-1.37 |
-0.35 |
-0.16 |
-0.92 |
-0.38 |
-0.4 |
|
| 908786 |
Eaf2 |
NM_134111 |
Mm.386836 |
MxBx |
910 |
transcription |
induction of apoptosis by intracellular signals;positive
regulation of transcription |
protein binding;transcriptional activator activity |
nucleoplasm;nucleus |
Eaf2 |
NM_134111 |
ELL associated factor 2 |
-0.61 |
-0.06 |
-0.20 |
-1.32 |
-0.33 |
-0.04 |
-1.61 |
-0.29 |
-0.61 |
|
| 914782 |
Trim30 |
NM_009099 |
Mm.295578 |
MAxI |
102 |
transcription |
protein ubiquitination;regulation of transcription,
DNA-dependent;transcription |
DNA binding;transcription factor activity;ubiquitin-protein
ligase activity;zinc ion binding |
cytoplasm;intracellular;nucleus;ubiquitin ligase complex |
Trim30 |
NM_009099 |
tripartite motif protein 30 |
0.12 |
0.21 |
0.13 |
0.19 |
0.09 |
0.66 |
1.31 |
0.50 |
0.35 |
|
| 413987 |
Cutl2 |
NM_007804 |
Mm.332661 |
MABI |
202 |
transcription |
regulation of transcription, DNA-dependent;transcription |
DNA binding;transcription factor activity |
nucleus |
Cutl2 |
NM_007804 |
cut-like 2 (Drosophila) |
0.61 |
0.27 |
0.55 |
2.28 |
0.72 |
0.60 |
2.61 |
1.24 |
0.6 |
|
| 570984 |
Abcg8 |
NM_026180 |
Mm.26581 |
MABI |
101 |
transport |
transport |
ATP binding;ATPase activity;ATPase activity, coupled to
transmembrane movement of substances |
integral to membrane;membrane |
Abcg8 |
NM_026180 |
ATP-binding cassette, sub-family G (WHITE), member 8 |
-0.29 |
0.24 |
0.01 |
-0.93 |
0.27 |
0.65 |
-1.65 |
0.32 |
-0.13 |
|
| 581260 |
M6prbp1 |
NM_025836 |
Mm.311696 |
MxBx |
613 |
transport (carbohydrate) |
|
receptor activity |
|
M6prbp1 |
NM_025836 |
mannose-6-phosphate receptor binding protein 1 |
-0.45 |
-0.01 |
-0.50 |
-1.06 |
0.02 |
-0.47 |
-0.79 |
-0.02 |
-0.68 |
|
| 626244 |
Cdw92 |
AK008866 |
Mm.270088 |
MxBI |
102 |
transport (choline) |
choline transport |
choline transporter activity |
integral to membrane |
NA |
AK008866 |
NA |
-0.61 |
-0.19 |
-0.21 |
-0.62 |
0.05 |
-0.28 |
-1.17 |
0.07 |
-0.11 |
|
| 929523 |
Txnrd3 |
NM_153162 |
Mm.229332 |
MxBx |
1313 |
transport (electron) |
electron transport;glutathione metabolism;thioredoxin pathway |
arsenate reductase (glutaredoxin) activity;disulfide
oxidoreductase activity;electron carrier activity;oxidoreductase
activity;thioredoxin-disulfide reductase activity |
cytoplasm;microsome |
Txnrd3 |
NM_153162 |
thioredoxin reductase 3 |
-0.13 |
0.52 |
0.41 |
-1.42 |
0.28 |
0.65 |
-0.69 |
0.32 |
-0.09 |
|
| 495744 |
Cyp2c37 |
NM_010001 |
Mm.220317 |
MxBI |
505 |
transport (electron) |
electron transport;transport |
monooxygenase activity;oxidoreductase activity;unspecific
monooxygenase activity |
endoplasmic reticulum;membrane;microsome |
Cyp2c37 |
NM_010001 |
cytochrome P450, family 2. subfamily c, polypeptide 37 |
-0.56 |
0.31 |
0.12 |
0.85 |
0.74 |
-0.54 |
2.24 |
-0.18 |
-0.65 |
|
| 737168 |
Ap1g2 |
NM_007455 |
Mm.238879 |
MABx |
102 |
transport (protein) |
endocytosis;intracellular protein transport;protein complex
assembly;protein transport;transport;vesicle-mediated transport |
binding |
Golgi apparatus;Golgi trans face;clathrin coat of trans-Golgi
network vesicle;coated pit |
Ap1g2 |
NM_007455 |
adaptor protein complex AP-1, gamma 2 subunit |
-0.64 |
0.00 |
-0.58 |
-1.04 |
-0.07 |
-0.49 |
-1.46 |
-0.90 |
-0.51 |
|
| 924107 |
Rab34 |
AF327929 |
Mm.275864 |
MxBx |
908 |
transport (protein) |
endocytosis;protein transport;small GTPase mediated signal
transduction;transport |
GTP binding;GTPase activity;guanyl nucleotide binding |
Golgi apparatus;early endosome;ruffle |
NA |
AF327929 |
NA |
-0.66 |
-0.34 |
-0.44 |
-1.06 |
-0.37 |
-0.75 |
-1.00 |
-0.82 |
-0.58 |
|
| 587635 |
Pop4 |
NM_025390 |
Mm.22284 |
MxBI |
102 |
tRNA processing |
tRNA processing |
hydrolase activity;ribonuclease P activity;ribonuclease
activity |
nucleus |
Pop4 |
NM_025390 |
processing of precursor 4, ribonuclease P/MRP family, (S.
cerevisiae) |
-0.60 |
0.39 |
-0.44 |
-1.57 |
0.10 |
-0.04 |
-0.22 |
0.01 |
0.18 |
|
| 928053 |
0610010O12Rik |
AK002512 |
Mm.272527 |
MxBx |
909 |
|
nucleus |
NA |
AK002512 |
NA |
-0.47 |
-0.02 |
-0.28 |
-1.25 |
0.09 |
-0.65 |
-1.45 |
-0.13 |
9E-03 |
|
| 596059 |
Angel1 |
NM_144524 |
Mm.63979 |
MxBx |
813 |
|
extracellular space |
1110030H02Rik |
NM_144524 |
RIKEN cDNA 1110030H02 gene |
-0.44 |
-0.11 |
-0.62 |
-1.57 |
-0.24 |
-0.71 |
-1.30 |
-0.35 |
-0.56 |
|
| 378072 |
2010208K18Rik |
NM_028095 |
Mm.28631 |
MxBI |
105 |
|
methyltransferase activity;transferase activity |
|
2010208K18Rik |
NM_028095 |
RIKEN cDNA 2010208K18 gene |
-0.47 |
-0.31 |
-0.62 |
-1.13 |
0.01 |
-0.32 |
0.01 |
-0.04 |
-0.64 |
|
| 806549 |
6330583M11Rik |
NM_024465 |
Mm.112632 |
MxBI |
102 |
|
catalytic activity |
|
6330583M11Rik |
NM_024465 |
RIKEN cDNA 6330583M11 gene |
-0.55 |
-0.15 |
-0.17 |
-0.98 |
-0.10 |
0.04 |
-0.59 |
-0.01 |
-0.13 |
|
| 530097 |
A530050D06Rik |
BC023353 |
Mm.77133 |
MxBx |
910 |
|
NA |
BC023353 |
NA |
-0.61 |
-0.20 |
-0.23 |
-1.30 |
-0.16 |
-0.40 |
-1.41 |
-0.08 |
-0.31 |
|
| 491149 |
Acrv1 |
AK030129 |
Mm.4776 |
MAxx |
101 |
|
acrosome;extracellular space |
Acrv1 |
AK030129 |
acrosomal vesicle protein 1 |
-0.01 |
0.38 |
0.38 |
0.06 |
0.49 |
0.43 |
1.79 |
1.05 |
1.03 |
|
| 580857 |
AU040829 |
NM_175003 |
Mm.134338 |
MABI |
101 |
|
AU040829 |
NM_175003 |
expressed sequence AU040829 |
-0.29 |
0.10 |
-0.20 |
-2.19 |
-0.12 |
-0.50 |
-1.58 |
-0.41 |
-0.03 |
|
| 854101 |
B230308G19Rik |
NM_173427 |
Mm.60400 |
MABI |
102 |
|
B230308G19Rik |
NM_173427 |
RIKEN cDNA B230308G19 gene |
-0.32 |
-0.37 |
-0.55 |
-2.26 |
-0.73 |
-1.04 |
-0.65 |
-0.60 |
-1.45 |
|
| 482840 |
BC027342 |
NM_146251 |
Mm.29046 |
MxBx |
1113 |
|
BC027342 |
NM_146251 |
cDNA sequence BC027342 |
-0.64 |
0.14 |
0.01 |
-1.24 |
-0.05 |
0.23 |
-1.35 |
0.12 |
-0.1 |
|
| 440174 |
BC061259 |
NM_198424 |
Mm.36852 |
MABx |
104 |
|
BC061259 |
NM_198424 |
cDNA sequence BC061259 |
-0.40 |
-0.38 |
-0.05 |
-1.29 |
-0.40 |
-0.36 |
-0.51 |
-0.26 |
-0.13 |
|
| 309451 |
Igtp |
NM_018738 |
Mm.33902 |
MxBx |
605 |
|
GTPase activity |
cytosol |
Igtp |
NM_018738 |
interferon gamma induced GTPase |
0.44 |
0.32 |
0.23 |
1.03 |
0.12 |
0.43 |
1.87 |
-0.01 |
0.48 |
|
| 420519 |
LOC209387 |
NM_199146 |
Mm.277377 |
MxBx |
301 |
|
LOC209387 |
NM_199146 |
tripartite motif protein 30-like |
0.20 |
-0.41 |
-0.25 |
0.53 |
0.61 |
0.10 |
1.69 |
-0.12 |
0.51 |
|
| 888038 |
LOC433243 |
AK077243 |
Mm.383335 |
MAxx |
101 |
|
NA |
AK077243 |
NA |
0.63 |
0.43 |
0.62 |
0.65 |
0.27 |
0.56 |
2.41 |
0.89 |
0.85 |
|
| 651122 |
Tusc3 |
NM_030254 |
Mm.272722 |
MxBx |
911 |
|
extracellular space;integral to membrane;mitochondrion |
Tusc3 |
NM_030254 |
tumor suppressor candidate 3 |
-0.67 |
-0.19 |
-0.41 |
-1.75 |
0.07 |
-0.49 |
-1.05 |
-0.07 |
-0.19 |
|
| 710465 |
Wdfy1 |
NM_027057 |
Mm.379166 |
MxBI |
105 |
|
Wdfy1 |
NM_027057 |
WD repeat and FYVE domain containing 1 |
0.11 |
0.00 |
-0.55 |
-0.82 |
-0.74 |
-0.27 |
-1.35 |
0.73 |
-0.62 |
|
| 509871 |
|
MxBI |
102 |
|
NA |
NA |
NA |
-0.66 |
-0.48 |
-0.58 |
-2.29 |
-0.24 |
0.19 |
-1.13 |
0.14 |
-0.4 |
|
| 532042 |
|
MxBx |
1013 |
|
NA |
NA |
NA |
-0.63 |
-0.40 |
-0.35 |
-1.93 |
0.02 |
-0.57 |
-1.82 |
0.75 |
-0.71 |
|
| 742311 |
|
MxBx |
1007 |
|
NA |
NA |
NA |
0.36 |
0.09 |
0.60 |
-0.72 |
0.01 |
1.26 |
-1.59 |
-0.17 |
0.58 |
|
| a
Each value represents the fold change (in log2
value) versus the non-treated control. |
|
| b
Eight ANOVA groups where A, B, I, x stands for
dose, time, interaction, and blank, respectively. |
|
|
| c
ANOVA cluster numbers classified to have
approximately 25 genes per class. |
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